A280 vs. protein assay?

Flip Hoedemaeker, CvF RUL/TNO, Leiden, The Netherlands sbtnfh at rulsfb.LeidenUniv.nl
Thu Sep 15 10:04:04 EST 1994


In article <tom-1509941445240001 at 131.211.54.137>, tom at radical.chm.jhu.edu (Tom Tullius) writes:
>Could dimerization somehow be "quenching" the
>absorbance at 280 nm? Ideas or experience would be appreciated.
>
>-- 
Only aromatic amino acids absorb at 280. Do you know if there are some on the
dimer interface? That could account for the quenching! I don't know if this
has been seen before, but it could be an explanation.

Flip 



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