A280 vs. protein assay?
Flip Hoedemaeker, CvF RUL/TNO, Leiden, The Netherlands
sbtnfh at rulsfb.LeidenUniv.nl
Thu Sep 15 10:04:04 EST 1994
In article <tom-1509941445240001 at 22.214.171.124>, tom at radical.chm.jhu.edu (Tom Tullius) writes:
>Could dimerization somehow be "quenching" the
>absorbance at 280 nm? Ideas or experience would be appreciated.
Only aromatic amino acids absorb at 280. Do you know if there are some on the
dimer interface? That could account for the quenching! I don't know if this
has been seen before, but it could be an explanation.
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