Renaturation of proteins from 8M Urea - references?

Patric Lundberg psl at ahabs.wisc.edu
Thu Sep 15 04:32:48 EST 1994


In article <DRESNICK.94Sep15133059 at m66-080-9.mit.edu>,
dresnick at athena.mit.edu (David I Resnick) wrote:

> Hi,
> 
> I'm expressing a ~130 amino acid protein fragment in E. coli.  After
> solubilization in 8 M Urea, I purify it using a His6 tag.  I then want
> to get rid of the urea (and pray that it will fold).  Everything I've
> tried so far (not many things, but a few) has resulted in
> precipitation.  Anyone out there know of a comprehensive reference for
> this sort of thing?  Any ideas???

Dialysis against 7, 6, 4, 2 M urea, then ddH2O?

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  psl at ahabs.wisc.edu
  UW-Madison, Mad-town USA

  Ma'let a"r ingenting, va"gen a"r allt - R. Broberg
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