tandrnawm at aol.com
Sun Sep 18 07:45:02 EST 1994
In article <323m3c$1ih at mserv1.dl.ac.uk>, <SPAGNOL at PLAUTO.CSATA.IT> writes:
I'm looking for a good protocol for deglycosilating a glycoprotein with
PNGase F. The one in Current Protocols for M.B. did not work in my hands
is also a little bit imprecise.For istance it says: add 1
e protein, w
ithout specifiyng in what should it be diluted. Pleas post to me by E-Mail
ill compile a summary. Thanks.
Sorry, Giorgio Spagnol
SPAGNOL at PLA
Try the Oxford GlycoSystems catalog. They show  of protein, PNGaseF,
and buffers. Alternatively, try the kit "GlycoShift", which uses PNGaseF.
Or, even, if you don't mind denaturing things, try trifluoromethane
sulfonic acid (TFMS), as in the GlycoFree kit from OGS.
[Disclaimer: I work for OGS]
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