2-D protein gels?

[Francis Ouellette]

Jim Owens (jow at helix.nih.gov) wrote:

> I am thinking of using 2-D electrophoresis to examine differences between
> two strains of mice under two sets of conditions.  My experience with
> protein gels ended about 15 years ago.  Could some kind soul suggest some
> references (books or articles) that could give me guidance in how to
> proceed?

Jim,

one place to look at is the Swiss-Prot web page, they have a
technical information on 2-D page with reciepes and all.

The URL for that page is:

http://expasy.hcuge.ch/  

(look at "SWISS-2DPAGE", and "Tecnical information" in the
Protocol section)

regards,

francis

--
| B.F. Francis Ouellette  
|
| francis at ncbi.nlm.nih.gov