Need help on IP.
gt3853a at prism.gatech.edu
Thu Sep 22 20:29:30 EST 1994
I have problem on regenerating PAS for IP( Immunoprecipitation). I use 0.3M Glycine (PH=3) to wash the used PAS for 4-5 times. But without adding any antibody, I still find the IgG band on the Westernblotting.
If you have any idea and like to share with people who work on this area .
Please response by e-mail. Thank you very much.
Georgia Institute of Technology, Atlanta Georgia, 30332
Internet: gt3853a at prism.gatech.edu
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