Affinity column needed..
int at ukc.ac.uk
Fri Sep 23 02:48:48 EST 1994
>Kim Hak-Sung (hskim at sorak.kaist.ac.kr) wrote:
>: Recently, I am trying to purify an enzyme which needs NAD+ as a cofactor.
>: I heard that there're some affinity columns which can be used for
>: this purpose.
>Cibacron Blue columns are used to bind proteins which have NAD as cofactor.
>There are lots of literature about this method (in about any book on protein
>purification there is a chapter dedicated to this topic), but for a start
> Earle Stellwagen: Chromatography on Immobilized Reactive Dyes.
> Meth. Enz. 182, 343-357, 1990.
Try writing to Affinity Chromatography ltd, they do a nice screening
kit called the PIKSI (Protein Isolation Kit for Sorbant Identification) which
contains 10 mimetic affinity dyes. I've used this to ID a good affinity ligand
for use in the purification of NADPH utilizing monooxygenases .
Their Technical inquiries address is
307 Huntigdon Road, Girton,
Cambridge, CB3 OJX, UK.
FAX England (0223) 277502
Hope this is of some use
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