gel retardation

WANZ at QUCDN.QueensU.CA WANZ at QUCDN.QueensU.CA
Sun Sep 25 10:40:41 EST 1994


Do you always expect one-band shift when you use gel retardation to
analyze drug-induced DNA-binding protein appearence(either existing
protein being modified or de novo protein synthesized)?
What are your comments on protein concentration caused multiple band
appearence?  Is this caused by protein polymerization or non-specific
DNA binding by other proteins?  When I changed total protein only 2 to 3
fold, I saw multiple bands appeared.
Your comments to this accounts is greatly appreciated.  Thank you.
Zhiwei Wang



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