Volatile buffers for lyophilization
sfredric at blue.weeg.uiowa.edu
Tue Sep 27 20:14:55 EST 1994
In note <36adsa$i7v at news.cs.tulane.edu>, jayuziuk at rs6.tcs.tulane.edu (Jeffrey
>Matt Parker (at332 at FreeNet.Carleton.CA) wrote:
>: We are currently using 20 mM ammonium bicarbonate as the
>: last buffer before lyophilization in our protein prep. Does anybody
>: have any suggestions for better buffers? We are having a hard time
>: getting all of the salt to evaporate.
>: Matthew Parker
>: Agriculture Canada
>I'm sorry if this is obvious, but how about ammonium hydroxide?
I usually use just 1mM ammonium bicarb. we have also used 1M acetic acid. . .
although, on a frequent basis, this is not very good for your pump.
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