Tricine for elecrophoresis

nussbaum at neurochem.u-strasbg.fr nussbaum at neurochem.u-strasbg.fr
Thu Sep 29 09:17:17 EST 1994


In article <fsco-280994095748 at dalessandro.nci.nih.gov> fsco at helix.nih.gov (Francesco D'Alessandro) writes:
>I am looking for a protocol to make and run a Tricine gel to resolve and
>isolate a 20KDa protein.
>Do you have any, or do you have a reference? Does this kind of gel present
>any problem in performing westerns?
>Thank you very much.
>Francesco

I have checked the  old handbook of my wife; I think the reference is 
correct; it concerns gel electrophoresis in the presence of tricine 
which worked in my hands ; I did also Western blots with WGA (lectin) 
detection without problems.
Reference: Schägger et Von Jagow , Analytical Biochemistry , vol 166, 
pages 368-379 (1987)



More information about the Proteins mailing list