Tricine for elecrophoresis
houthaeve at embl-heidelberg.de
Fri Sep 30 07:50:20 EST 1994
In article <fsco-280994095748 at dalessandro.nci.nih.gov>, fsco at helix.nih.gov (Francesco D'Alessandro) writes:
> I am looking for a protocol to make and run a Tricine gel to resolve and
> isolate a 20KDa protein.
> Do you have any, or do you have a reference? Does this kind of gel present
> any problem in performing westerns?
> Thank you very much.
The best reference paper for tricine gels is :
H. Schaegger, G. von Jagow, 1987. Analytical Biochemistry 166, 368-379.
Tank blotting does not give problems if you pre-equilibrate your gel long
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