IgM

Fabio Nunes fnunes at pop.nih.gov
Fri Sep 30 08:35:16 EST 1994


I'm using a monoclonal IgM produced by a company. This antibody labels
filaments in CV1 and other epithelial cells.  I'm trying to isolate what
seems to be a new protein. When I run Western blot I obtain 3 bands with
this antibody. I'm wondering if that result could be a cross reaction. Does
anyone have experience in isolate proteins using IgM monoclonal antibodies.
Any help would be greatly appreciated.
Thanks in advance

Fabio Nunes
NIDCD-NIH
fnunes at pop.nih.gov





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