Disappearing SDS-PAGE bands

Kelly Ambler kelly at hearts.bsd.uchicago.edu
Fri Dec 1 18:35:48 EST 1995


Hi netters-

I recently had some very strange results from my Coumassie stained 
gels.  The proteins in the middle of the gels "disappeared".  The 
bands at the bottom half of the gel and at the very top of the gel 
appeared to run as usual.  This occured with more than one gel - all 
poured at the same time, but electrophoresed separately.  Has anyone 
else encountered this?  Does anyone know what causes this?  The only 
hypothesis I can come up with is "aliens" :-)

Thanks in advance.

Kelly Ambler
University of Chicago




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