protease deficient strains

Ding Ming ming at ahabs.wisc.edu
Wed Feb 1 10:33:22 EST 1995


In article <3glq0s$d2b at news.tamu.edu>, mary at bio.tamu.edu (Mary Fernandes) wrote:

> I am experiencing severe degradation problems with the expression of a
> fungal DNA-binding protein in E.coli. I have tried both GST and His tag
> systems and have also tried to make a truncated protein, but the
> problem persists. Does anyone have a strain that is lon- or lon-htpr
> that I could use for this purpose? Any suggestions are welcome. Thanks.
> 

This is an interesting thread and please post your answer to the net. Thanks.
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Dr.Ding Ming
University of Wisconsin-Madison
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