Anti phosphoserine/theonine Antibodies

Gary Krause gkrause at cms.cc.wayne.edu
Tue Feb 28 13:48:20 EST 1995


In article <01HNKCNINJZ6A5U6L6 at MPGARS.DESY.DE> DREWES at MPASMB.DESY.DE writes:
>From: DREWES at MPASMB.DESY.DE
>Subject: Re: Anti phosphoserine/theonine Antibodies
>Date: 27 Feb 1995 16:37:29 -0800


>Subject: Anti- phospho serine/ threonine Antibodies
>in <1995Feb27.203818.29299 at oxvaxd>
>golding at sable.ox.ac.uk (Dr Simon Golding) wrote:

>> I am currently using an antiphosphotyrosine - HRP conjugated antibody
>> in Western blots which is supplied by Transduction Labs. Does anybody
>> know of a source of anti-phospho- serine/threonine antibodies
>> (HRP/AP conjugated or not)?

>> Thanks for your help, Simon.

>Anti P-Ser and P-Thr antibodies are available from Sigma. However, they are
>quite new and I have not used them yet. It would be very interesting to know
>if they are comparable in specifity and affinity to the very good anti P-Tyr
>antibodies which are now commercially available from different companies. 
>Maybe anybody else can comment on this?

>Gerard Drewes
>Max Planck Unit for Structural Molecular Biology
>DESY, Hamburg 22603, Germany


We are using the Sigma anti-phosphoserine on nitrocellulose blots.  Horrible 
backgrounds.  You can't use Blotto or nonfat dry milk to block since they both 
contain serine-phosphorylated proteins.  5% Tween 20 is ineffectual as is 10% 
BSA.  I would love to hear if anybody has solved this problem.

Gary


-------------------------------------------
Gary Krause (gkrause at cms.cc.wayne.edu)
Department of Emergency Medicine
Wayne State University, Detroit, MI USA



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