reprobe western blots

Fiberman william at neuro.usc.edu
Thu Jan 5 01:17:13 EST 1995


Hello, Netters,

Can a western blot filter be "reprobed" like a Southern blot filter?  If
so, what is the best way to strip the original antibody?  I am using a
secondary antibody conjugated to alkaline phosphatase, and visualizing
with addition of color substrates NBT and BCIP.  I would appreciate your
response.

-fm




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