coupling peptides

[Hallick Lab]

	I have a question about coupling peptides to carrier proteins for use in
immunizations.  I am interested in using EDC to couple my peptides to BSA.  I
would like to know people's opinion of this method.  Is it tricky?  Is it a
fairly successful method?  I am worried about blocking free amino groups on
the peptide.  Is this necessary?  (Each of my 10mer peptides has at least two
internal lysines.)  I would appreciate any hints or comments.
Thanks,
Kristin Jenkins
Dept. of Molecular and Cellular Biology
University of Arizona
hallicklab at tikal.biosci.arizona.edu