reprobe western blots

anon anon
Thu Jan 5 18:43:18 EST 1995


In article <3eg2t9$8hl at neuro.usc.edu>, william at neuro.usc.edu (Fiberman) wrote:

> Hello, Netters,
> 
> Can a western blot filter be "reprobed" like a Southern blot filter?  If
> so, what is the best way to strip the original antibody?  I am using a
> secondary antibody conjugated to alkaline phosphatase, and visualizing
> with addition of color substrates NBT and BCIP.  I would appreciate your
> response.
> 
> -fm

   I think this method causes a precipitate to form, and you can't strip
and reprobe when using this method.
 
 
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