reprobe western blots

davis at wehi.edu.au davis at wehi.edu.au
Thu Jan 5 17:29:20 EST 1995


In article <3eg2t9$8hl at neuro.usc.edu>, william at neuro.usc.edu (Fiberman) writes:
> 
> Hello, Netters,
> 
> Can a western blot filter be "reprobed" like a Southern blot filter? 

Yes, it can.  Use the following stripping buffer:  100 mM 2-ME, 2% SDS, 62.5 mM
Tris.HCl pH 6.7.  Submerge membrane in buffer at 50 deg C for 30 minutes.  Wash
twice for 10 minutes in your usual wash buffer.  Reblock.  Reprobe.

I have used this several times and it works.

Ian Davis					davis at licre.ludwig.edu.au
Ludwig Institute for Cancer Research
Melbourne Tumour Biology Branch
Melbourne, Australia.




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