Digital chromatograms: parts list?

Jim Bassuk Bassuk at U.Washington.Edu
Fri Jan 6 02:55:26 EST 1995


In standard chromatography of proteins, we monitor the absorbance of the column eluate with a
Pharmacia UV-1 flow cell coupled to a chart recorder.  This analog signal from the UV-1 is 10 mV.

To design a system to convert the signal to a digital format, I'll need a A/D converter, some sort
of board for a yet-to-be purchased 486 PC, and some sort of software to acquire, process, and store
the data.

We'll likely couple two UV-1 units in tandem (214 and 280 nm).....this is 2 channels.....and then
a simple closure switch to indicate the advancement of the fraction collector....this is another channel...
a total of 3 channels.....

No need to buy a big, expensive HPLC software program, because I won't be controlling any pumps, 
etc...

Comments on boards, A/D units, and software sources and experiences are respectfully requested.

Thanks in advance,

Jim Bassuk
BioStructure
Univ Washington



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