chicken antibodies

Nora Plesofsky-Vig npv at mani.unet.umn.edu
Tue Jan 10 14:30:08 EST 1995


I made antibodies in chickens and have isolated the IgGs from egg  
yolks, but I was not entirely pleased with the results. I had high  
background reactivity of the pre-immune yolk IgGs from the three  
chickens I used, and it was therefore absolutely essential to include  
a control reaction with the pre-immune IgGs in all experiments.  
However, this problem may depend on the conditions under which the  
soon to be immunized chickens are kept, and an affinity isolation of  
the specific IgGs should get around the problem. 


Also, if you are considering immunoprecipitation with the antibodies,  
you should be aware that chicken IgGs do not react with protein A or  
protein G, which therefore cannot be used to amplify the  
immunoprecipitation (unless you use an anti-chicken IgG antibody from  
mammals first, as I did). However, there are now other tools on the  
market that may get around this problem. Promega (Madison, Wisc)  
offers an immobilized anti-chicken IgY (IgG) for immunoprecipitation,  
which may be useful.

Good luck.

Nora Plesofsky-Vig
nora at molbio.cbs.umn.edu



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