Crossed Immunoelectrophoresis

C Webster mphcw at gps.leeds.ac.uk
Tue Jan 10 08:54:58 EST 1995


Does anyone have experience of using crossed immunoelectrophoresis for the 
study of glycoforms of protein ?

I am currently working on the microheterogenity of acid glycoprotein using
concanvalin A in the first dimension but having problems in getting the method
to work. I think my problem is the first dimension i.e. with the con A.

Does anyone have a method for using con A in CIE with regard to buffers etc.

I would appreciate any help on this matter.

TIA


-- 
Mr Craig Webster, Department of Chemical Pathology and Immunology
The Old Medical School, Thoresby Place, Leeds, LS2 9JT
Tel:- 0532 923285                    email:- craigw at pathology.leeds.ac.uk
Fax:- 0532 335672                            mphcw at gps.leeds.ac.uk



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