TCA-precipitation?

Ekramoddoullah, Abul Kalam M. aekramoddoul at PFC.Forestry.CA
Wed Jan 11 19:10:34 EST 1995


In article <3euc3i$3ib at sunserver.lrz-muenchen.de>, u7q11aa at sunmail.lrz-muenchen.de (Peter Alliger) writes:
>I am looking for good method to precipitate a highly purified protein
>before 
>analysing it on a SDS-PAGE.
>The fraction has about 1M KCl and should be concentrated from 500 ul to
>about 20.
>
>Thanks for suggestions
>
>PIT
>
>===============================================================
>Peter Alliger, Ph.D.                   
>Institute for Immunology                 home address:
>University of Munich                    
>Goethestr. 31                           Maximilian-Wetzger-Str.7
>D-80336 Munich, Germany                 D-80636 Munich, Germany
>
>Phone:(49-89)5996-673                   (49-89)183426
>Fax:  (49-89)5160-2236
>----------------------------------------------------------------
>  
Why not prcipitate with 8X volume of cold acetone (at -20C) for a 1 h and
redissolve the ppt in sample buffer required for SDS-PAGE. Good Luck. Abul 

  EKRAMODDOULLAH, ABUL KALAM M.     Title: Research Scientist
  Forestry Canada                   Phone: (604) 363-0600
  Victoria, B.C.                    Internet: AEKRAMODDOUL at A1.PFC.Forestry.CA



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