pET vectors and low yield

Gilles VACHON vachon at bio.grenet.fr
Mon Jan 16 04:53:13 EST 1995


Dear netters,

I am having trouble in getting regular amounts of pET (19b or 11a, with ot
without insert)) in DH5alpha.
I have only 30-50ug of plasmid from a 200ml LB culture  (Cesium Chloride prep).

The DH5alpha strain is ok since I get 500-700ug of pUC in the same conditions.

Does anyone on the net have an answer to that problem ?
I am planning to do cloning with these pETs and I bet I won't be able to
check the clonings with that low yield.

Thanks
Dr. Gilles Vachon
Laboratoire de Biologie Moleculaire Vegetale
CERMO, 3eme etage
Universite J. Fourier, BP 53X
38041 GRENOBLE CEDEX
FRANCE
Tel: (33) 76 51 48 92
fax: (33) 76 51 43 36
e-mail: vachon at bio.grenet.fr




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