pET vectors and low yield

Cornelius Krasel krasel at alf.biochem.mpg.de
Mon Jan 16 10:01:21 EST 1995


Gilles VACHON (vachon at bio.grenet.fr) wrote:
> I am having trouble in getting regular amounts of pET (19b or 11a, with
> or without insert) in DH5alpha.
> I have only 30-50ug of plasmid from a 200ml LB culture  (Cesium Chloride 
> prep).

I'm afraid that's normal. I have the same problems with pET3* in JM109.
(pET isn't exactly a high-copy plasmid ...)

--Cornelius.

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/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany  */
/* email: krasel at alf.biochem.mpg.de                 fax: +49 89 8578 3795  */
/* "Science is the game you play with God to find out what His rules are." */



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