pET vectors and low yield

Cornelius Krasel krasel at
Mon Jan 16 10:01:21 EST 1995

Gilles VACHON (vachon at wrote:
> I am having trouble in getting regular amounts of pET (19b or 11a, with
> or without insert) in DH5alpha.
> I have only 30-50ug of plasmid from a 200ml LB culture  (Cesium Chloride 
> prep).

I'm afraid that's normal. I have the same problems with pET3* in JM109.
(pET isn't exactly a high-copy plasmid ...)


/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany  */
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