pET vectors and low yield

[Cornelius Krasel]

Gilles VACHON (vachon at bio.grenet.fr) wrote:
> I am having trouble in getting regular amounts of pET (19b or 11a, with
> or without insert) in DH5alpha.
> I have only 30-50ug of plasmid from a 200ml LB culture  (Cesium Chloride 
> prep).

I'm afraid that's normal. I have the same problems with pET3* in JM109.
(pET isn't exactly a high-copy plasmid ...)

--Cornelius.

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