Protein elution from Ni-NTA resin

Jim Bassuk Bassuk at U.Washington.Edu
Sun Jan 15 21:07:31 EST 1995


In article <3f6ae3$od6 at usenet.INS.CWRU.Edu>, pxb33 at po.CWRU.Edu (Parkash Badola) says:
>
>
>Hi friends, I am interested in knowing if there is
>some alternative method of eluting the poly His
>tagged protein bound to Ni-NTA resin in addition to
>using Imidazole or changing pH.
>Thanks
>Parkash Badola

Sure.....try 10 mM EDTA or 10 mM DTT....both reagents will remove your
protein from the column (although the nickel ion will come with)..

Jim Bassuk
BioStructure
UWashington



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