pET vectors and low yield

Benoit_Hebert at IAF.UQUEBEC.CA Benoit_Hebert at IAF.UQUEBEC.CA
Mon Jan 16 23:37:22 EST 1995


Gilles VACHON (vachon at bio.grenet.fr) wrote:
> I am having trouble in getting regular amounts of pET (19b or 11a, with ot
> without insert)) in DH5alpha.
> I have only 30-50ug of plasmid from a 200ml LB culture  (Cesium Chloride prep).

> The DH5alpha strain is ok since I get 500-700ug of pUC in the same conditions.

As Cornelius pointed out, this is normal. Indeed, I get similar amounts with
pMal-c2. I did get better results in XL-1 Blus when compared to TB1. I haven`t 
tried the DH5alpha yet, I will during the next week. I suggest to amplify your
production with chloramphenicol.

Benoit

========================================
Benoit_Hebert at iaf.uquebec.ca

Virology Research Center
Armand-Frappier Institute, Laval, Quebec
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