SDS-PAGE problem

ANON r.k.mcewen at bham.ac.uk
Tue Jan 17 18:50:01 EST 1995


Recently I`ve been observing an artefactual band on my SDS-PAGE gels 
regardless of whether there is any samples on it. This band typically runs 
between 50 and 60 kDa right across the gel, through all the sample lanes and 
varys in intensity from one to another.
 To date, I have tried fresh reagents; a colleagues reagents and gel equipment 
all to no avail.
Needless to say, he does not see this band. I have tried to mimic his method 
of preparing the gel but I am at a loss to explain it.

Any suggestion ?



More information about the Proteins mailing list