SDS-PAGE problem

sb9e sb9e at faraday.clas.virginia.edu
Tue Jan 17 17:13:58 EST 1995


In article <r.k.mcewen.30.0012D5FE at bham.ac.uk>
r.k.mcewen at bham.ac.uk (ANON) writes:

> Recently I`ve been observing an artefactual band on my SDS-PAGE gels 
> regardless of whether there is any samples on it. This band typically runs 
> between 50 and 60 kDa right across the gel, through all the sample lanes and 
> varys in intensity from one to another.
>  To date, I have tried fresh reagents; a colleagues reagents and gel equipment 
> all to no avail.
> Needless to say, he does not see this band. I have tried to mimic his method 
> of preparing the gel but I am at a loss to explain it.
> 
> Any suggestion ? 

Wear gloves, filtrate solutions just before pouring, and, magically,
this human keratin band will disappear forever from your gels. 

 Serge
sb9e at virginia.edu



More information about the Proteins mailing list