Protein elution from Ni-NTA resin

graeser at ubaclu.unibas.ch graeser at ubaclu.unibas.ch
Wed Jan 18 13:18:04 EST 1995


In article <3f6ae3$od6 at usenet.INS.CWRU.Edu>, pxb33 at po.CWRU.Edu (Parkash Badola) writes:
> Hi friends, I am interested in knowing if there is
> some alternative method of eluting the poly His
> tagged protein bound to Ni-NTA resin in addition to
> using Imidazole or changing pH.
> Thanks
> Parkash Badola

Try 50 mM EDTA, it will elute everything including the Ni++ from your cloumn.
You can reload your column with Ni++ using a 2% NiSo4 solution.
        Hope that helps
			Ralph Graeser



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