SDS-PAGE problem

mark damico mwdst1 at pitt.edu
Fri Jan 20 14:25:58 EST 1995


In article <r.k.mcewen.30.0012D5FE at bham.ac.uk>, r.k.mcewen at bham.ac.uk
(ANON) wrote:

> Recently I`ve been observing an artefactual band on my SDS-PAGE gels 
> regardless of whether there is any samples on it. This band typically runs 
> between 50 and 60 kDa right across the gel, through all the sample lanes and 
> varys in intensity from one to another.
>  To date, I have tried fresh reagents; a colleagues reagents and gel
equipment 
> all to no avail.
> Needless to say, he does not see this band. I have tried to mimic his method 
> of preparing the gel but I am at a loss to explain it.
> 
> Any suggestion ?

Yes. Try not to touch anything, including your stock solutions, without
wearing gloves. You are probably seeing bands that correspond to the
keratins from your fingers.

-- 
mwd



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