SDS-PAGE problem

ryan at ryan at
Wed Jan 18 17:50:48 EST 1995

In article <r.k.mcewen.30.0012D5FE at>, r.k.mcewen at (ANON) writes:
> Recently I`ve been observing an artefactual band on my SDS-PAGE gels 
> regardless of whether there is any samples on it. This band typically runs 
> between 50 and 60 kDa right across the gel, through all the sample lanes and 
> varys in intensity from one to another.
>  To date, I have tried fresh reagents; a colleagues reagents and gel equipment 
> all to no avail.
> Needless to say, he does not see this band. I have tried to mimic his method 
> of preparing the gel but I am at a loss to explain it.
> Any suggestion ?

Maybe you could elaborate a little: how are you visualizing your bands? 
Does your unwanted band show up only after staining, or is it radioactive,
or is it visible without staining in the gel itself? Is in confined to 
gel lanes, or does it also show in the spaces between lanes? Do you always run
the sample loading buffer dye-front off the gel?

	Kevin W. Ryan
	Department of Virology & Molecular Biology
	St. Jude Children's Research Hospital
	Memphis, Tennessee 38101-0318, U.S.A.
	phone: (901) 522-0411
	fax:   (901) 523-2622
	Internet: ryan at

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