Help: UV difference spectra

VOSS at AIMP1.UNA.AC.AT VOSS at AIMP1.UNA.AC.AT
Mon Jan 23 07:22:19 EST 1995


Hi out there,
I am trying to interpret the UV-difference spectra of a native enzyme
with two differently denatured states. One state is obtained at pH 3.0, the 
other by removing a Zn atom which seems to be essential for stabilizing the 
native state.
In both cases I get nice peaks in the difference spectrum (denat - native) 
at 299 and 286 nm, respectively. The first  spectrum, however is negagtive, 
the second positive.
The appearance of the peaks tell me that the only Trp residue seems to get 
into a more hydrophobic environment. 
But what does the fact of getteing either a positive or a negative peak 
tells me?
Is there any good literature available dealing with this matter?

Thanks for any help!


Tilman
 
*********************************************************
Tilman Voss Ph.D.
Protein Chemistry Group
Bender+Co
Vienna, Austria
Tel: xx43-1-80105 351
Fax: xx43-1-80105 366
Email: VOSS at AIMP.UNA.AC.AT
*********************************************************




More information about the Proteins mailing list