inclusion bodies / protein folding

Nataly Koutsia lpnatali at weizmann.weizmann.ac.il
Mon Jan 23 11:05:00 EST 1995


In article <knierman.6.000534BA at fcrfv1.ncifcrf.gov> Michael D. Knierman,
knierman at fcrfv1.ncifcrf.gov writes:
>Does anyone have any experience 1. working with recombinant protein
inclusion
>bodies from E. Coli? 2. trying to refold denatured proteins to regain
function?
>
>and does anybody have any references in general for refolding denatured
>proteins to regain activity?
>
>Thanks


Dear Michael! I did solubilisation of inclusion bodies and it worked
fine. Now I trying to refold my protein. I may give you protocol that I
have and I would be glad
to know how it will work for you.
Best luck.
                      Nataly.


Solubilization of inclusion bodies


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