inclusion bodies / protein folding

Nataly Koutsia lpnatali at
Mon Jan 23 11:05:00 EST 1995

In article <knierman.6.000534BA at> Michael D. Knierman,
knierman at writes:
>Does anyone have any experience 1. working with recombinant protein
>bodies from E. Coli? 2. trying to refold denatured proteins to regain
>and does anybody have any references in general for refolding denatured
>proteins to regain activity?

Dear Michael! I did solubilisation of inclusion bodies and it worked
fine. Now I trying to refold my protein. I may give you protocol that I
have and I would be glad
to know how it will work for you.
Best luck.

Solubilization of inclusion bodies

More information about the Proteins mailing list