Help: anti peptide antibodies

Ekramoddoullah, Abul Kalam M. aekramoddoul at PFC.Forestry.CA
Mon Jan 23 16:53:25 EST 1995


In article <1995Jan19.124928.1 at mcvax4.d48.lilly.com>, djohnson at mcvax4.d48.lilly.com writes:
>It's been a while since I've conjugated peptides for immunization.  Are there
>kits available to make it easier?   Where should I look?  Thanks, Dave.

During the synthesis of your peptide, add cysteine at the C-terminal end and
then conjugate to KLH or other protein carriers (containing SH group) via
Maleimidobenoyl-N-hydroxysuccinimide ester (MBS). I understand Pierce Chemical
has a kit. I hope this would help. Abul

  EKRAMODDOULLAH, ABUL KALAM M.     Title: Research Scientist
  Forestry Canada                   Phone: (604) 363-0600
  Victoria, B.C.                    Internet: AEKRAMODDOUL at A1.PFC.Forestry.CA



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