Antibodies to phosphorylated amino acids

Cornelius Krasel krasel at alf.biochem.mpg.de
Thu Jan 26 07:41:12 EST 1995


Sarah Swanson (swanson at nature.berkeley.edu) wrote:
> I'm looking at the possibility of using antibodies to phosphorylated amino
> acids as an alternative to radiolabelling in studies of protein
> phosphorylation. Has anyone got any tips/comments about this, especially
> regarding the specificity of the antibodies.

Phosphotyrosine antibodies apparently work well. There is a phosphoserine
/ -threonine antibody on the market (Sigma) but AFAIK its specificity is
lousy.

--Cornelius.

--
/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany  */
/* email: krasel at alf.biochem.mpg.de                 fax: +49 89 8578 3795  */
/* "Science is the game you play with God to find out what His rules are." */



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