Antibodies to phosphorylated amino acids

Gary Krause gkrause at cms.cc.wayne.edu
Fri Jan 27 10:41:22 EST 1995


In article <3ga5j2$omq at emory.mathcs.emory.edu> rmiller at bimcore.emory.edu (Richard Miller) writes:
>From: rmiller at bimcore.emory.edu (Richard Miller)
>Subject: Re: Antibodies to phosphorylated amino acids
>Date: 27 Jan 1995 06:58:42 GMT



I can echo the lack of specifity of Sigma's phosphoserine antibody.  We use 
brain tissue and we get a large number of bands that represent non-specific 
staining.  Another problem is background blocking.  Using non-fat fry milk is 
impossible since it contains phosphoserine proteins.  Using only tween-20 
gives horrible backgrounds.  We are trying BSA/tween-20 which helps some, but 
we are nowhere near publishable quality pictures.


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Gary Krause (gkrause at cms.cc.wayne.edu)
Department of Emergency Medicine
Wayne State University, Detroit, MI USA



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