HPLC Assay Question

Carl Hixon cjhixon at netcom.com
Mon Jan 30 19:15:30 EST 1995


Does anybody know of literature regarding measuring Human Albumin and 
Fibrinogen concentration using HPLC?  Secondly, I have a protein 
suspension containing small protein particles (few microns in diameter)  
These particles are constructed of Albumin and Fibrinogen.  Would it be 
possible to determine the protein concentration of this type of 
suspension using HPLC by digesting the particles?  I worry about the 
different sizes of the protein fragments.  If anybody has literature 
regarding these issues please forward the references.  Thank you!

Carl



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