Do proteins self-digest?

Abiye Iyo aiyo at uoguelph.ca
Mon Jan 30 16:21:43 EST 1995


Hi, 
I recently used the GST fusion system to purify my protein (a 
cellulase).  Everything went fine up to the stage of gel filteration and 
I had a nice single band .  Now the misery part is that my protein which 
is about 57kD degrades to two low molecular weight products which are 
still active on zymograms.  Does this mean my prep is contaminated with 
proteases or I am experiencing some form of self digestion judging from 
the fact that my protein was pure initially?  And how can I prevent 
this?  I need help before my patience runs out.

Abiye





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