Best E.coli strains for GST-expression (re. minimum proteolysis)
krasel at alf.biochem.mpg.de
Mon Jan 30 16:34:14 EST 1995
Jim Litts (jlitts at onyx-pharm.com) wrote:
[asking for E. coli strains with protease deletions]
BL21 and derivatives are E. coli B strains which are protease-deficient
compared to WT E. coli K-12 (e.g. they do not contain outer membrane
There are several lon-negative E. coli K-12 strains. lon is an ATP-
dependent protease. K-12 which are deficient in the heat-shock
response (sorry, can't recall genotype) are also lon-deficient.
Some of these strains are available from NEB.
/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany */
/* email: krasel at alf.biochem.mpg.de fax: +49 89 8578 3795 */
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