Best E.coli strains for GST-expression (re. minimum proteolysis)

Cornelius Krasel krasel at alf.biochem.mpg.de
Mon Jan 30 16:34:14 EST 1995


Jim Litts (jlitts at onyx-pharm.com) wrote:

[asking for E. coli strains with protease deletions]

BL21 and derivatives are E. coli B strains which are protease-deficient
  compared to WT E. coli K-12 (e.g. they do not contain outer membrane
  proteases)
There are several lon-negative E. coli K-12 strains. lon is an ATP-
  dependent protease. K-12 which are deficient in the heat-shock
  response (sorry, can't recall genotype) are also lon-deficient.
  Some of these strains are available from NEB.

--Cornelius.

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/* Cornelius Krasel, Abt. Lohse, Genzentrum, D-82152 Martinsried, Germany  */
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/* "Science is the game you play with God to find out what His rules are." */



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