protease deficient strains

Mary Fernandes mary at
Tue Jan 31 11:54:52 EST 1995

I am experiencing severe degradation problems with the expression of a
fungal DNA-binding protein in E.coli. I have tried both GST and His tag
systems and have also tried to make a truncated protein, but the
problem persists. Does anyone have a strain that is lon- or lon-htpr
that I could use for this purpose? Any suggestions are welcome. Thanks.

Mary Fernandes
mary at

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