simultaneous extraction of DNA, RNA, and proteins

Stanley Hum hum at
Mon Jul 3 12:01:05 EST 1995

Has anyone used TRIZOL reagent (from GIBCO) to extract DNA, RNA and proteins from small tissue samples (in the order of 80 to 100 micrograms)?

If not TRIZOL, is there any other method of simultaneous extractions of small amounts of tissue besides fluorometric analysis (using ethidium bromide)?

I would like to use a spectrophotometer since a fluorometer is a rare item in our labs.

any help would be appreciated!



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