Triton-X 100 effect on protein concentration determination
dkim at nmsu.edu
Fri Jul 7 12:56:41 EST 1995
I have been reading this thread, and thought this might be relevant:
I am using a protein determination kit called dotMETRIC. It is a
spot-blot kind of assay, in which the diameter of the spot on a gel is
proportional to the total amount of protein. Thus, a single spot of 1
microliter of unknown protein solution can be used to get a reasonably
accurate measure of total protein. The manufacturers claim that the
assay is unaffected by detergents, and does not require a protein
standard curve. It gives the same result regardless of the individual
I am new at this protein game (Proteins have always been the stuff I
throw away in the phenol phase), so I thought the kit would be worth a
try. It does seem to be very easy and consistent. I have made no formal
comparisons with other assay methods, however. Has anyone else tried
this kit and made a comparison?
If interested, here is the address, etc for the manufacturer:
Geno Technology, Inc.
3803 Washington Blvd.
St. Louis, MO 63108 USA
FAX (314) 534-1883
Daniel Kim (no affiliation with the company.)
More information about the Proteins