Triton-X 100 effect on protein concentration determination

U12201 at uicvm.uic.edu U12201 at uicvm.uic.edu
Fri Jul 7 13:14:46 EST 1995


The Pierce BCA assay works like a charm even in the presence of very
high conc. of TX100.
Any Coomassie type assay (Bradford) will give high backgrounds values
with TX100. No way around it!!
I personally prefer BCA for its versatility and flexibility of the
protocol (you can "tune" the sensitivity by varying the time  and
the temp.)
KeldS at uic.edu



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