Triton-X 100 effect on protein concentration determination

Anne Spang spang at vms.biochem.mpg.de
Sat Jul 8 04:40:59 EST 1995


Hi!
Triton X-100 messes up your Bradford assay! In the presence of detergent
in our sample, we usually precipitate the sample with TCA in a dotblot
apparatus on nitrocellulose, then stain with amido black. After elution
teh samples can be read in an ELISA reader or a normal photometer.

Hope this helps
      Anne



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