Hydrophobic protein purification troubles

Wolfgang Schechinger u7k0201 at sunmail.lrz-muenchen.de
Sun Jul 9 16:08:41 EST 1995


>> More troublesome is that the fusion of the 
>> same protein to the maltose-binding protein 
>> (MBP) is sticking to the walls of the plastic
>> eppendorf tubes after HPLC purification.
>
>More pure a hydrophobic protein the more will be its propencity to bind 
>to plastic (hydrophobic) surfaces. Try siliconizing.
>
Doesn't siliconizing meking the surface even more hydrophobic?

I would try a glass tube instead.

Wolfgang




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