Triton-X 100 effect on protein concentration determination

[Bob Litt]

In article <spang-0807951142340001 at genmac8.biochem.mpg.de>,
spang at vms.biochem.mpg.de (Anne Spang) wrote:

> Hi!
> Triton X-100 messes up your Bradford assay! In the presence of detergent
> in our sample, we usually precipitate the sample with TCA in a dotblot
> apparatus on nitrocellulose, then stain with amido black. After elution
> teh samples can be read in an ELISA reader or a normal photometer.
> 
> Hope this helps
>       Anne

I have heard that the BioRad DC assay is supposed to be compatible with
triton X-100.  Has anyone tried this, and does it work as advertised?

Bob Litt

-- 
r-litt at uchicago.edu
Segev Lab
Department of Molecular Genetics and Cell Biology
University of Chicago