metal peptide coordination

Andrew, Tel. +396-91093434 WALLACE at IRBM.IT
Tue Jul 11 13:20:45 EST 1995

Hi Len,  
I have to post this here as a reply to your own address bounced.

Anyway, zinc fingers might be one possibility to look at - zinc ion
coordination is required for their DNA-binding domains to fold correctly. The
relevant binding domains are stable as shortish peptides (20-30 aas long) and
display a rapid cooperative transition from a random coil to a 2-stranded-beta
sheet - alpha-helical structure upon binding to metal ion, which can be
followed by circular dichroism and UV-spectroscopy (the latter if cobalt is
used instead of zinc). Look for papers by Jeremy Berg (a Medline search should
turn him up) and there are also several X-ray and NMR structures of zinc
fingers and steroid hormone receptors (which use similar domains) in the PDB
database where you can extract geometrical information.

All the best,
             Andrew Wallace, IRBM P. Angeletti, Pomezia, Italy.

                           Voice: +39-6-91093434
                           Fax:   +39-6-91093225
                           Email: wallace at

Discussion on phage display, combinatorial libraries, etc. - molreps at
                    DISCLAIMER: I speak only for myself.

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