metal peptide coordination

Andrew, Tel. +396-91093434 WALLACE at IRBM.IT
Tue Jul 11 13:20:45 EST 1995


Hi Len,  
I have to post this here as a reply to your own address bounced.

Anyway, zinc fingers might be one possibility to look at - zinc ion
coordination is required for their DNA-binding domains to fold correctly. The
relevant binding domains are stable as shortish peptides (20-30 aas long) and
display a rapid cooperative transition from a random coil to a 2-stranded-beta
sheet - alpha-helical structure upon binding to metal ion, which can be
followed by circular dichroism and UV-spectroscopy (the latter if cobalt is
used instead of zinc). Look for papers by Jeremy Berg (a Medline search should
turn him up) and there are also several X-ray and NMR structures of zinc
fingers and steroid hormone receptors (which use similar domains) in the PDB
database where you can extract geometrical information.

All the best,
Andrew
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             Andrew Wallace, IRBM P. Angeletti, Pomezia, Italy.

                           Voice: +39-6-91093434
                           Fax:   +39-6-91093225
                           Email: wallace at irbm.it

Discussion on phage display, combinatorial libraries, etc. - molreps at irbm.it
                    DISCLAIMER: I speak only for myself.
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