metal peptide coordination
Andrew, Tel. +396-91093434
WALLACE at IRBM.IT
Tue Jul 11 13:20:45 EST 1995
I have to post this here as a reply to your own address bounced.
Anyway, zinc fingers might be one possibility to look at - zinc ion
coordination is required for their DNA-binding domains to fold correctly. The
relevant binding domains are stable as shortish peptides (20-30 aas long) and
display a rapid cooperative transition from a random coil to a 2-stranded-beta
sheet - alpha-helical structure upon binding to metal ion, which can be
followed by circular dichroism and UV-spectroscopy (the latter if cobalt is
used instead of zinc). Look for papers by Jeremy Berg (a Medline search should
turn him up) and there are also several X-ray and NMR structures of zinc
fingers and steroid hormone receptors (which use similar domains) in the PDB
database where you can extract geometrical information.
All the best,
Andrew Wallace, IRBM P. Angeletti, Pomezia, Italy.
Email: wallace at irbm.it
Discussion on phage display, combinatorial libraries, etc. - molreps at irbm.it
DISCLAIMER: I speak only for myself.
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