Q Ni-NTA resin

QIAGEN QIAGEN at kaiwan.com
Tue Jul 11 17:14:04 EST 1995


We noticed an error in the posting of our Message-ID:
<QIAGEN-070795092431 at 192.0.2.1>

It should read:

Ni-NTA resin can probably be reused after stripping the column with EDTA
and recharging it with Ni ions. However, this will give you no guarantee
that you have removed all the residual proteins and you risk contamination
of your next prep. Also, the resin properties will progressively
deteriorate as the level of residual contamination increases.

The long washing procedure we describe is tedious, we know, but it is the
only way to be absolutely sure you have removed all contaminants (both
protein and non-protein) that may be interacting with the resin. Most users
find that it is safer, more convenient, and more cost-effective (unless you
are using extremely high resin volumes) to use fresh Ni-NTA resin where
possible. 

Also, you should note that Ni-NTA resin will bind 5 to 10 mg of protein per
ml
                                                                           
            
                                                   ^^^^^^
of resin, and that the purification procedure is more efficient if you use
less resin than you actually need, and overload it somewhat. The QIAGEN
Technical Service Department has extensive references for this system, and
can help you optimize your purification procedure by using less Ni-NTA
resin.

QIAGEN Technical Service Department
800-426-8157



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