Help! clogged HIC cartridge

RossN at imb.lan.nrc.ca RossN at imb.lan.nrc.ca
Fri Jul 14 15:10:04 EST 1995


Have you tried loading a pronase (0.5 mg/ml in buffer at pH7.5) solution and
letting the column sit in that for 24-48 h at 37 C then flush with buffer
(+/-SDS?)? This worked for me (-SDS) with reverse phase HPLC columns that
were clogged with clam proteins. Column resolution was restored. Note: when
doing this run the column backwards as suggested by another responder.
 
Goo luck (;
 
Neil W. Ross, Ph.D.
National Research Council Canada
Institute for Marine Biosciences
1411 Oxford St., Halifax, NS  B3H 3Z1
(902)426-8402     FAX (902) 426-9413
Email: ROSSN at imb.lan.nrc.ca
 
 
 
 
 
 



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