in vitro disulfide formation

Michael D. Knierman knierman at ncifcrf.gov
Fri Jul 14 23:48:35 EST 1995


rbateman at wave.st.usm.edu (Dr. Robert Bateman) wrote:

>I have a small (100 aa) protein with a single cysteine which is active
>as a protease inhibitor in the monomer form. I am wondering if it is 
>inactive in the disulfide-bonded dimeric form. I would like some suggestions
>on how to get it to form intermolecular disulfide bonds so I can test this.

>Bob Bateman
>rbateman at wave.st.usm.edu

I used to cyclize synthetic peptides by disolving them in a buffered
solution above pH 7.5 and letting them stir for a day or so.  The
disolved oxygen oxidized the Cys residues into disulfides.




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